Chromatographic Techniques in Forensic Science

Chromatographic Techniques in Forensic Science Quiz Crafted By-

Prerna Patel

Team Member

Sherlock Institute of Forensic Science India Pvt. Ltd, New Delhi

Introduction to Chromatographic Techniques in Forensic Science   

Chromatography is the process by which compounds within a mixture are separated. This can be achieved by properties such as size, and how the compounds interact with the mobile and solid phases of chromatography. There are different types of chromatography, ranging from 2D-based (planar) methods such as Thin Layer Chromatography (TLC) and column-based methods, including Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC).

Let's look at the questions and their answer. 

Ques 1. Chromatography was first reported by:

a) Ramsey

b) Henry

c) Pascal

d) Leduce

 Answer- a) Ramsey

Ques 2. Thin Layer Chromatography is______

a) Partition Chromatography                                                      

b) Electrical mobility of ionic species

c) Adsorption chromatography                                                      

d) None of the above

Answer- c) Adsorption chromatography

Ques 3.  In gas chromatography, the basis for separation of the components of the volatile material is the difference in _______

a) Partition coefficients                                                      

b) Conductivity

c) Molecular weight                                                      

d) Molarity

Answer- a) Molecular weight 

Ques 4.  In thin layer chromatography, the stationary phase is made of _______ and the mobile phase is made of _______

a) Solid, liquid                                                  

b) Liquid, liquid

c) Liquid, gas                                                        

d) Solid, gas

Answer- a) Solid, liquid 

Ques 5.  Which compounds are held by the stationary phase?

a) Polar compound                                                  

b) Non-Polar compound

c) a and b both                                                        

d) None of the above

Answer- a) Polar compound

Ques 6. Thermal conductivity Detector in Gas Chromatography consists of _______ identical filaments.

a) Two                                                      

b) Four

c) Six                                                        

d) Eight

Answer- b) Four

Ques 7.  The most suitable solvent for thin layer chromatography/ paper chromatography of inks is:

a)  N-butanol: pyridine: water (3:1:1.5)                                                    

b) Amyl alcohol: acetic acid: chloroform (6:1:2)

c) Ethanol: acetone: acetic acid (4:1:5)                                                       

d) Amyl alcohol: acetic acid: pyridine (6:1:2)

Answer- a) N-butanol: pyridine: water (3:1:1.5) 

Ques 8. What is the Analyte?

a) Substance for separation                                                   

b) Substance for impurity

c) a & b                                                        

d) none of the above

Answer- a) Substance for separation

Ques 9.  Which technique is also known as color writing?

a) NMR                                                      

b) Mass spectroscopy

c) Chromatography                                                        

d) All of the above

Answer- c) Chromatography 

Ques 10. Chromatography is a physical method that is used to separate ________ 

a) Simple mixtures                                                      

b) Complex mixtures

c) Viscous mixtures                                                        

d) Metals

Answer- b) Complex mixtures